کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8478665 | 1551149 | 2013 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
BMP2 and GDF5 induce neuronal differentiation through a Smad dependant pathway in a model of human midbrain dopaminergic neurons
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کلمات کلیدی
GDF5Dorsomorphinnumber of repetitionsRT-PCRTGFPBSGDNFday(s) in vitroBMP2MAPK - MAPKventral midbrain - midbrain وسترنMitogen activated protein kinases - Mitogen فعال پروتئین کینازMTT - MTTtransforming growth factor - تبدیل فاکتور رشدThiazolyl blue tetrazolium bromide - تترازولیم برومید تیزولیل آبیNeuronal differentiation - تمایز نورونdopaminergic/dopamine - دوپامینرژیک / دوپامینDIV - دیوSH-SY5Y cells - سلول های SH-SY5YGlial cell line-derived neurotrophic factor - فاکتور نوروتروفی مشتق از سلول گلیاییPhosphate buffered saline - فسفات بافر شورReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایBMP receptor - گیرنده BMP
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Parkinson's disease is the second most common neurodegenerative disease, and is characterised by the progressive degeneration of the nigrostriatal dopaminergic (DA) system. Current treatments are symptomatic, and do not protect against the DA neuronal loss. One of the most promising treatment approaches is the application of neurotrophic factors to rescue the remaining population of nigrostriatal DA neurons. Therefore, the identification of new neurotrophic factors for midbrain DA neurons, and the subsequent elucidation of the molecular bases of their effects, are important. Two related members of the bone morphogenetic protein (BMP) family, BMP2 and growth differentiation factor 5 (GDF5), have been shown to have neurotrophic effects on midbrain DA neurons both in vitro and in vivo. However, the molecular (signalling pathway(s)) and cellular (direct neuronal or indirect via glial cells) mechanisms of their effects remain to be elucidated. Using the SH-SH5Y human neuronal cell line, as a model of human midbrain DA neurons, we have shown that GDF5 and BMP2 induce neurite outgrowth via a direct mechanism. Furthermore, we demonstrate that these effects are dependent on BMP type I receptor activation of canonical Smad 1/5/8 signalling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 56, September 2013, Pages 263-271
Journal: Molecular and Cellular Neuroscience - Volume 56, September 2013, Pages 263-271
نویسندگان
Shane V. Hegarty, Aideen M. Sullivan, Gerard W. O'Keeffe,