کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8479425 | 1551319 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inducibility of doxycycline-regulated gene in neural and neuroendocrine cells strongly depends on the appropriate choice of a tetracycline-responsive promoter
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کلمات کلیدی
TETEF1α promoterDLRrtTATTATTSTet-OnHCMVDOXtetracycline operator - اپراتور تتراسایکلینInducible gene expression - بیان ژن قابل انعطافtetracycline repressor - تتراسایکلین سرکوبگرTetracycline - تتراسایکلینtetr - تترانtetO - تتوtetracycline-controlled transactivator - ترانسکتیواتور تحت کنترل تتراسایکلینdoxycycline - داکسی سایکلینNeural cells - سلول های عصبیNeuroendocrine cells - سلولهای نوروآندوکرینHuman cytomegalovirus - سیتومگالوویروس انسانی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Elucidation of the mechanisms underlying specific receptor activation of neural and neuroendocrine cells will require the establishment of cellular systems that permit the regulation of the expression of the protein of interest. In a tetracycline (Tet)-regulated system, the gene encoding the protein of interest is under the control of a Tet promoter and its transcription is activated in the presence of doxycycline (Dox) by the Tet transactivator rtTA. Acceptable inducibility of the gene's expression requires a high level of its expression in the presence of Dox and a minimal basal expression in the absence of Dox. Two Tet promoters are compared here, the original PhCMV*-1 and the second-generation Ptight, with respect to the inducibility of the gene of interest in neuroendocrine and neural cells genetically engineered to express rtTA, namely PC12-Tet-On cells and MB-G-18 cells (mouse brain-derived cells with the phenotype of neuron-restricted precursors). This study demonstrates that the use of Ptight provided a much higher Dox-induced maximal expression in both cell lines, while the basal activities of the two Tet promoters were at similar levels. The additional use of the Tet-controlled silencer (tTS) caused almost complete abrogation of the leakiness of the Ptight promoter and an increase in the inducibility of the regulated gene, but the maximal levels of gene expression driven in the presence of Dox were also markedly reduced.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 52, Issues 1â2, January 2008, Pages 221-229
Journal: Neurochemistry International - Volume 52, Issues 1â2, January 2008, Pages 221-229
نویسندگان
Dagmara Klopotowska, Leon Strzadala, Janusz Matuszyk,