کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8482602 | 1551533 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of a Standard Newborn Screening Test for the Rapid Diagnosis of Inhibited Ã-Oxidation in Atypical Myopathy in Horses
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Use of a Standard Newborn Screening Test for the Rapid Diagnosis of Inhibited Ã-Oxidation in Atypical Myopathy in Horses Use of a Standard Newborn Screening Test for the Rapid Diagnosis of Inhibited Ã-Oxidation in Atypical Myopathy in Horses](/preview/png/8482602.png)
چکیده انگلیسی
Atypical myopathy (AM) in horses is caused by ingestion of seeds or seedlings of some maple trees (Acer species, sapindaceae family). Elevated concentrations of acyl conjugates, a sign of inhibited Ã-oxidation of fatty acids, are diagnostic for AM. Quantification of acyl conjugates is currently not part of the standard test scheme of veterinary laboratories but is done routinely in laboratories screening human newborns for inborn errors of metabolism. It was our aim to show that the screening method, which is based on the use of whole blood dried on filter paper, can be applied to samples from horses with the single methodological adaptation, that is, spotting serum instead of whole blood on the filter paper. Acylcarnitines (ACs) were measured by tandem mass spectrometry after butylation. Results were compared with those obtained by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in the corresponding liquid samples. Inhibition of fatty acid Ã-oxidation was unequivocally shown by elevated concentrations of butyryl-/isobutyrylcarnitine, valeryl-/isovalerylcarnitine, and hexanoylcarnitine (intraday coefficient of variation < 20%) in horses affected by AM. To be able to use the existing analytic tools and methods of the screening laboratories, no attempt was made to include the toxins and their metabolites in the test. Rapid diagnosis of inhibited Ã-oxidation indicating AM in horses is possible by a routine test available in pediatric screening laboratories.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Equine Veterinary Science - Volume 67, August 2018, Pages 71-74
Journal: Journal of Equine Veterinary Science - Volume 67, August 2018, Pages 71-74
نویسندگان
Johannes Sander, Michael Terhardt, Stefanie Sander, Nils Janzen,