کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8502767 | 1554053 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of calpain-1 activity and protein proteolysis by protein nitrosylation in postmortem beef
ترجمه فارسی عنوان
مقررات فعالیت پروتئولیز پروتئاز پروتئین کلپین-1 با پروتئین نیتریزیل در گوسفند بعد از قاعدگی
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
The effects of nitric oxide (NO) and its induced protein nitrosylation on calpain-1 activation and protein proteolysis in beef during postmortem aging were investigated. Five semimembranosus muscles were removed from beef cattle carcass. Beef samples were incubated with one of following treatments for 24â¯h at 4â¯Â°C: control (normal saline), NO donor (100, 200 and 400â¯Î¼Mâ¯S-nitrosoglutathione (GSNO)) or nitric oxide synthase (NOS) inhibitor (0.05, 0.1 and 0.15â¯M NÏ-Nitro-l-arginine methyl ester hydrochloride (L-NAME)). After incubation, the beef samples were vacuum-packaged and aged at 4â¯Â°C for 1, 4, and 7â¯days. Results showed that GSNO decreased and L-NAME increased the extent of calpain-1 autolysis at d 1. Degradation of desmin and troponin-T was increased by L-NAME while decreased by GSNO. These results suggest that NO could regulate calpain-1 autolysis and its proteolysis activity during postmortem aging in beef SM muscle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 141, July 2018, Pages 44-49
Journal: Meat Science - Volume 141, July 2018, Pages 44-49
نویسندگان
Chaoyang Zhang, Rui Liu, Anran Wang, Dacheng Kang, Guanghong Zhou, Wangang Zhang,