کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8504096 | 1554325 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of two adhesins of Actinobacillus seminis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of two adhesins of Actinobacillus seminis Identification of two adhesins of Actinobacillus seminis](/preview/png/8504096.png)
چکیده انگلیسی
Actinobacillus seminis is an autochthon of ovine reproductive organs. However, it is also considered to be a pathogen of these animals and is responsible for causing epididymitis and low fertility, among other pathological diseases. In this study, we describe the identification of two proteins functioning as adhesins in A. seminis. After obtaining surface proteins of this bacterium, two proteins of approximately 25 and 40âkDa were partially purified by ion interchange chromatography. Both proteins interacted with bovine fibronectin and fibrinogen, and they cross-reacted with a polyclonal serum from a sheep infected with A. seminis. Both proteins were identified by mass spectrometry analysis as phosphoglycerate mutase and elongation factor-Tu, respectively. A. seminis was able to adhere to cultured human bladder cells, and pre-incubation of A. seminis with the polyclonal sera against these proteins significantly inhibited their ability to adhere to cells but adherence was not affected by pre-immune serum, indicating their activity as adhesins. The identification of proteins involved in the adhesion of A. seminis to tissues and that serve as virulence factors will help furtherour understanding of the pathogenic mechanisms of this bacterium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 167, October 2018, Pages 100-103
Journal: Small Ruminant Research - Volume 167, October 2018, Pages 100-103
نویسندگان
J. Fernando Montes-GarcÃa, Delil A. Chincoya Martinez, Sergio Vaca Pacheco, Candelario Vázquez Cruz, Patricia Sanchez Alonso, Juan Xicohtencatl Cortes, Hector Trujillo-Ruiz, Erasmo Negrete-Abascal,