کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8504719 | 1554987 | 2018 | 47 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The scavenger receptor CD163 is widely used as a cell signature for alternatively active “M2” macrophages in mammals. In this study, we generated two monoclonal antibodies, ABM-1A9 and ABM-2D6, against the extracellular region of bovine CD163. Conventional Western blotting using the antibodies yielded immunoreactive bands of bovine CD163 at 130 and 150â¯kDa in non-reduced and reduced spleen lysates, respectively. The minimum limit of detectable concentration of both antibodies was relatively lower (5.0â¯ng/mL) than that of the anti-human CD163 monoclonal antibody AM-3â¯K (>1.0â¯Î¼g/mL), which has been used previously for the detection of bovine CD163. An immunohistochemical study using formalin-fixed paraffin-embedded sections revealed that ABM-1A9 and ABM-2D6 clearly stained some Iba1+ macrophages in the lymph nodes of cattle with mastitis. Moreover, the CD163-stained macrophages were frequently observed engulfing leukocytes. ELISA using ABM-2D6 distinguished levels of circulating soluble CD163 in healthy cattle (less than 16.9â¯pmol/mL) and cattle with mastitis (more than 33.7â¯pmol/mL). These new monoclonal antibodies can be used in the diagnosis and evaluation of inflammatory disease prognosis in cattle with immunohistological analyses and blood test applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 198, April 2018, Pages 6-13
Journal: Veterinary Immunology and Immunopathology - Volume 198, April 2018, Pages 6-13
نویسندگان
Yoshinori Shimamoto, Junko Nio-Kobayashi, Hiroshi Watarai, Masashi Nagano, Natsuko Saito, Eiki Takahashi, Hidetoshi Higuchi, Atsushi Kobayashi, Takashi Kimura, Hiroshi Kitamura,