Modified temporal-phase-unwrapping method for measuring in real time the out-of-plane displacements of the tympanic membrane of Mongolian Gerbil

A technique for measuring in real-time continuous out-of-plane displacements of delicate objects is proposed, and demonstrated on the tympanic membrane of Mongolian Gerbil. The technique is based on the combination of two methods: the spatial phase shifting (SPS) and the modified temporal phase unwrapping (TPU). The combination allows to obtain, in several steps, the phase values of the points that undergo out-of-plane displacement as the object is deformed. The technique reduces the frame acquisition time of the standard TPU used in moiré interferometry by a factor of 4, which is important to diminish post-mortem artifacts during in-vitro experiments and to reduce motion artifacts in in-vivo tests. The proposed technique is robust against problems associated with the temporal phase-shifting method, such as nonlinear phase shift and noise. The advantages and disadvantages are discussed.