کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
852689 909409 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beyond nanosizing: an approach to shape analysis of fluorescent nanostructures by SMI-microscopy
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی (عمومی)
پیش نمایش صفحه اول مقاله
Beyond nanosizing: an approach to shape analysis of fluorescent nanostructures by SMI-microscopy
چکیده انگلیسی

Using spatially modulated illumination (SMI) light microscopy it is possible to measure the sizes of fluorescent structures that have an extension far below the conventional optical resolution limit (“subresolution size”). Presently, the sizes are determined as the object extension along the optical axis of the SMI microscope. For this, however, “a priori” assumptions on the fluorochrome distribution (“shape”) within the examined fluorescent structure have to be made. Usually it is assumed that the fluorochrome follows a Gauss-distribution or a spherical distribution. In this report we overcome the necessity to make an assumption on the shape of the fluorochrome distribution. We introduce two new experimentally obtained parameters which allow the determination of a shape measure to describe the spatial distribution of the fluorescent dye. This becomes possible by independent measurements with different excitation wavelengths. As an example, we present shape parameter measurements on individual fluorescent microspheres with a nominal geometrical diameter (“size”) of 190 nm. In the case investigated, the experimental shape correlated well with a homogeneous fluorochrome distribution (“spherical shape”) but not with a variety of other “shapes”.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Optik - International Journal for Light and Electron Optics - Volume 117, Issue 1, 15 January 2006, Pages 26–32
نویسندگان
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