کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8528910 | 1558847 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
TNF-alpha and TNF-R1 regulate bupivacaine-induced apoptosis in spinal cord dorsal root ganglion neuron
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: TNF-alpha and TNF-R1 regulate bupivacaine-induced apoptosis in spinal cord dorsal root ganglion neuron TNF-alpha and TNF-R1 regulate bupivacaine-induced apoptosis in spinal cord dorsal root ganglion neuron](/preview/png/8528910.png)
چکیده انگلیسی
Local anesthesia has been shown to render severe spinal cord neurotoxicity. This study used an in vitro model to explore the expression and function of tumor necrosis factor (TNF) signaling pathway in bupivacaine-induced apoptotic injury in spinal cord dorsal root ganglia (DRG). DRG was prepared from adult C57BL/6 mice and incubated with 10â¯mM bupivacaine in vitro to induce apoptosis. QRT-PCR and western blot demonstrated that bupivacaine upregulated TNF-alpha (TNF-α) and TNF receptor 1 (TNF-R1), but left TNF receptor 2 (TNF-R2) unaffected in DRG. SiRNA-mediated TNF-α or TNF-R1 inhibition, but not TNF-R2 inhibition, rescued bupivacaine-induced DRG apoptosis. In addition, qRT-PCR and western blot demonstrated that downstream substrates of apoptotic and TNF signaling pathways, caspase-9, MAP3K and JNK, were all significantly downregulated by TNF-α or TNF-R1 inhibition, but not by TNF-R2 inhibition, in bupivacaine-injured DRG. Thus, our work suggested that TNF-α and TNF-R1 are the major contributors of TNF signaling pathway in anesthesia-induced spinal cord neurotoxicity. Targeting TNF-α / TNF-R1, not TNF-R2 signaling pathway may be the key component to rescue or prevent anesthesia-induced apoptotic injury in spinal cord neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 833, 15 August 2018, Pages 63-68
Journal: European Journal of Pharmacology - Volume 833, 15 August 2018, Pages 63-68
نویسندگان
Ke Zhang, Shuai Yang, Chaozhi Luo,