کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
853471 | 1470686 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In-fiber Photo-immobilization of Bioactive Surfaces: An Optimization Study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی (عمومی)
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چکیده انگلیسی
Bioactive surface formation within photonic crystal fibers (PCFs) was demonstrated via photochemical means in our earlier work. The bioactive surface consisted of streptavidin bound to a biotin-functionalized layer of protein. Here, we address the issue of non-specific binding between streptavidin molecules and any exposed silica surfaces within the PCF. Increasing the protein coverage mitigated this problem, where a denser coverage was achieved by repeating the protein passivation. Subsequent study on improving the specific binding was also performed by increasing the duration of photobleaching, which resulted in more photochemically bound biotin. Optimal parameters were then selected based upon practicality and repeatability of results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Procedia Engineering - Volume 140, 2016, Pages 166-170
Journal: Procedia Engineering - Volume 140, 2016, Pages 166-170
نویسندگان
Derrick Yong, Elizabeth Lee, Kwang Yong Lee, Xia Yu, Chi Chiu Chan, Quan Liu,