کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8542571 1561450 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of RP-HPLC method for simultaneous estimation of Montelukast Sodium and Fexofenadine hydrochloride in combined dosage form
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی فارماکولوژی، سم شناسی و اقلام دارویی (عمومی)
پیش نمایش صفحه اول مقاله
Development and validation of RP-HPLC method for simultaneous estimation of Montelukast Sodium and Fexofenadine hydrochloride in combined dosage form
چکیده انگلیسی
A simple, fast and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous determination of Montelukast Sodium (MONT) and Fexofenadine hydrochloride (FEXO). The chromatographic separation was achieved on X-bridge C18 column (250 mm × 4.6 mm, 5 μm) as stationary phase with a mobile phase comprising of 50 mM Sodium acetate buffer:acetonitrile:methanol (25:35:40) adjust pH 8.2 with 5% o-phosphoric acid at a flow rate of 1.0 mL/min, column temperature of 40 ± 2 °C and UV detection at 210 nm. The retention time of Montelukast Sodium and Fexofenadine hydrochloride were 3.43 min, and 8.22 min respectively. The linearity were found to be in the range of 12.5-37.5 μg/mL and 150-450 μg/mL for Montelukast Sodium and Fexofenadine hydrochloride with correlation coefficient greater than 0.999. The proposed methods were validated as per ICH guidelines and successfully applied for the determination of investigated drugs in tablets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmacy Research - Volume 6, Issue 1, January 2013, Pages 134-139
نویسندگان
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