کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8542581 | 1561450 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simple RP-HPLC method for estimation of diazepam in tablet dosage form
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
فارماکولوژی، سم شناسی و اقلام دارویی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Simple RP-HPLC method for estimation of diazepam in tablet dosage form Simple RP-HPLC method for estimation of diazepam in tablet dosage form](/preview/png/8542581.png)
چکیده انگلیسی
A simple reverse phase HPLC method was developed and validated for the determination of diazepam present in pharmaceutical dosage form. A Hypersil ODS C-18 column (250 Ã 4.6 mm, packed with 5 microns) is used as stationary phase. An isocratic mode with mobile phase consisting of acetonitrile, methanol and 1% phosphate buffer (pH-3) in ratio of 18:58:24 (v/v/v) at a flow rate of 1 ml/min and effluent was monitored at 232 nm. Chromatogram showed a peak of DZP at retention time of 6.23 ± 0.002 min. The linearity range was found to be 2-20 μg/ml. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation, robustness and ruggedness. Recovery of DZP was found to be in the range of 99.4-100.3%. The limit of detection and limit of quantitation for estimation of DZP was found to be 0.898 μg/ml and 2.72 μg/ml, respectively. Proposed method was successfully applied for the quantitative determination of DZP in pharmaceutical dosage forms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmacy Research - Volume 6, Issue 1, January 2013, Pages 140-144
Journal: Journal of Pharmacy Research - Volume 6, Issue 1, January 2013, Pages 140-144
نویسندگان
A. Sruthi, P. Tejaswi, N. Thanuja, D. Sudheer Kumar, P. Vivek Sagar,