کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8548116 | 1561734 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cholesterol modulates the liposome membrane fluidity and permeability for a hydrophilic molecule
ترجمه فارسی عنوان
کلسترول، سیالیت و نفوذپذیری غشای لیپوزوم را برای یک مولکول هیدروفیلی مدول می کند
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کلمات کلیدی
کلسترول، سیالیت، لیپوزوم ها، مدل های ریاضی، نفوذپذیری، رهایی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
The effect of cholesterol (CHOL) content on the permeability and fluidity of dipalmitoylphosphatidylcholine (DPPC) liposome membrane was investigated. Liposomes encapsulating sulforhodamine B (SRB), a fluorescent dye, were prepared by reverse phase evaporation technique (REV) at various DPPC:CHOL molar ratios (from 100:0 to 100:100). The release kinetics of SRB was studied during 48â¯h in buffer (pH 7.4) containing NaCl at 37â¯Â°C. The DPPC:CHOL formulations were also characterized for their size, polydispersity index and morphology. Increasing CHOL concentration induced an increase in the mean liposomes size accompanying with a shape transition from irregular to nanosized, regular and spherical vesicles. The release kinetics of SRB showed a biphasic pattern; the release data was then analyzed using different mathematical models. On the overall, the SRB release was governed by a non-Fickian diffusion during the first period (0-10â¯h) while it followed a Fickian diffusion between 10 and 48â¯h. Changes in DPPC liposome membrane fluidity of various batches (CHOL% 0, 10, 20, 30 and 100) were monitored by using 5- and 16 doxyl stearic acids (DSA) as spin labels. CHOL induced a decrease in the bilayer fluidity. Concisely, CHOL represents a critical component in modulating the release of hydrophilic molecules from lipid vesicles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 113, March 2018, Pages 40-48
Journal: Food and Chemical Toxicology - Volume 113, March 2018, Pages 40-48
نویسندگان
Samar Kaddah, Nathalie Khreich, Fouad Kaddah, Catherine Charcosset, Hélène Greige-Gerges,