کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8548303 | 1561735 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of in vitro genotoxic effects of an anti-diabetic drug sitagliptin
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کلمات کلیدی
MMCNDICASSCGESCEsGLP-1GIPT2DMmitomycin-CDPP-4Chromosome aberrations - اختلالات کروموزومیSingle Cell Gel Electrophoresis - الکتروفورز ژل تک سلولیsister chromatid exchanges - تبادل کروماتید خواهرAntidiabetic drug - داروهای ضد دیابتیbinucleate - دو هسته ایType 2 diabetes mellitus - دیابت نوع دوDipeptidyl peptidase-4 - دیپپتیدیل پپتیداز 4Genotoxicity - سمیت ژنتیکیSitagliptin - سیتاگلیپتینnuclear division index - شاخص تقسیم هسته ایreplication index - شاخص تکثیرmitotic index - شاخص متیوتیکHuman lymphocytes - لنفوسیت های انسانیMicronucleus - میکروسکوپglucose-dependent insulinotropic polypeptide - گلیکول وابسته به پلی پپتید انسولینوتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sitagliptin is an active ingredient of antidiabetic drug used in the treatment of type 2 diabetes mellitus (T2DM). In this study, the genotoxic effects of sitagliptin were determined in human lymphocytes by using chromosome aberrations (CAs), sister chromatid exchanges (SCEs), micronucleus (MN) and comet assays. 31.25-1000â¯Î¼g/mL concentrations of sitagliptin were used. Sitagliptin significantly increased the frequency of CAs and SCEs at the highest concentration at 24â¯h treatment and all concentrations (except 250â¯Î¼g/mL for CA, except 31.25 and 62.50â¯Î¼g/mL for SCE) at 48â¯h treatment compared with solvent control (DMSO). This compound increased the MN at only the highest concentration compared with the solvent control. Mitotic index (MI) significantly decreased at the three highest concentrations of sitagliptin at 48â¯h treatment. However, replication (RI) and nuclear division (NDI) indices were not affected at all the treatments. Comet assay results indicated that sitagliptin significantly increased mean comet tail intensity and tail moment at only two concentrations (62.50 and 1000â¯Î¼g/mL for intensity, 125 and 1000â¯Î¼g/mL for tail moment), and tail length at all concentrations (except 125 and 500â¯Î¼g/mL). It was concluded that higher concentration of sitagliptin had genotoxic effects in the human lymphocytes in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 112, February 2018, Pages 235-241
Journal: Food and Chemical Toxicology - Volume 112, February 2018, Pages 235-241
نویسندگان
Deniz Yuzbasioglu, Cemile Enguzel-Alperen, Fatma Unal,