Osteogenic Differentiation of Adipose-derived Mesenchymal Stem Cells into Polycaprolactone (PCL) Scaffold

The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showed that cell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showed that cell-seeded PCL scaffolds cultured in osteogenic medium presented significantly greater amount of staining dye when compared to basal medium and scaffold without cells. The PCL scaffold have great potential to be used associated to ADSCs in Tissue Engineering to bone tissue regeneration