کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
858186 | 1470739 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proof of Principle of a Novel Impedance Microbiology Method Based on Bacteriophages Functionalized Paramagnetic Nanobeads
ترجمه فارسی عنوان
اثبات اصل یک روش میکروب شناسی امپدانس رمان بر اساس نانوذرات پارامغناطیس کارکردی باکتریوفاژها
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موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی (عمومی)
چکیده انگلیسی
An unconventional approach to impedance microbiology for Escherichia coli detection is under investigation. The detection principle of solution conductivity variation is based on intracellular content escape resulting from bacteriophage cell lysis. Bare electrodes on-chip included in a PDMS chamber were applied to the impedance measurements. Proofs of principle experiments were performed. In parallel, paramagnetic nano-beads were functionalised with selective phages for sample magnetic concentration and future methods integration. The system potential detection limit is about 10 CFU/chamber and provides the means for selective detection of viable cells. The methods integration could provide cost–effective results in less than 1 hour.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Procedia Engineering - Volume 87, 2014, Pages 328-331
Journal: Procedia Engineering - Volume 87, 2014, Pages 328-331