کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8588831 1564798 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran
چکیده انگلیسی
Coagulase is considered as a major determinant factor for the identification of Staphylococcus aureus strains. The 3′-end coding region of the coagulase (coa) gene contains a series of 81-bp tandem repeats, which differ in the number and location of enzymatic restriction sites among different isolates. coa PCR-RFLP has been used widely to type S. aureus isolates in epidemiological studies. The current study was conducted to investigate the coagulase gene polymorphisms in S. aureus isolated from various food samples using an in house PCR-RFLP method. A total of 100 strains of S. aureus were isolated from food samples. Isolates were typed by PCR-RFLP analysis using NdeI restriction digestion of the coagulase gene PCR products. Results showed that amplification of coagulase genes from S. aureus produced different PCR products. The isolates were grouped into 18 genotypes using RFLP analysis results of the genes. In this study, the S. aureus isolates have been shown to include more than one coagulase genotype, but only had a few coa genotypes predominated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Science and Human Wellness - Volume 5, Issue 4, December 2016, Pages 186-190
نویسندگان
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