Effects of CXCL3 on migration, invasion, proliferation and tube formation of trophoblast cells

Immunofluorescence staining demonstrated that CXCL3 localized in both trophoblasts of placenta and HTR-8/SVneo cells. Moreover, data showed that migration, invasion, proliferation and tube-formation capability of HTR-8/SVneo cells transfected with siRNA-CXCL3 were suppressed by down-regulation of CXCL3, while those behaviors of HTR-8/SVneo cells transfected with pEZ-CXCL3 were enhanced by upregulation of CXCL3. Nevertheless, the apoptosis of HTR-8/SVneo cells was not affected by neither siRNA nor overexpression plasmid. The result suggests that CXCL3 is involved in migration, invasion, proliferation and tubule formation of trophoblasts and may play a key role in the pathogenesis of preeclampsia.