کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644425 | 1569759 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LPS-induces IL-6 and IL-8 gene expression in bovine endometrial cells “through DNA methylation”
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کلمات کلیدی
methyl CpG-binding protein 2LPSIL-6qRT-PCRIL-8BSPMecp2TLR4cDNA - cDNAComplementary DNA - DNA تکمیلیBisulfite sequencing PCR - PCR توالی بیسولفیتinterleukin 6 - اینترلوکین 6Interleukin 8 - اینترلوکین 8ELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاlipopolysaccharide - لیپوپلی ساکاریدMethylation - متیلاسیونToll-like receptor 4 - گیرنده تله مانند 4
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Uterine infection in dairy cows causes great economic loss. In bovine endometrial cells, lipopolysaccharide (LPS)-stimulated increase in interleukin 6 (IL-6) and interleukin 8 (IL-8) mRNA is crucial for the inflammatory response; however, the regulatory mechanisms remain unclear. Here, we investigated the role of DNA methylation in IL-6 and IL-8 mRNA expression following LPS-induction in bovine endometrial cells. IL-6 and IL-8 mRNA expression was evaluated under DNA methylation inhibition using 5-Aza-2â²-deoxycytodine (5Aza) following LPS stimulation. Expression of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), methyl CpG-binding protein 2 (MeCP2) and DNA methylation at IL-6 and IL-8 regions, were analyzed using quantitative real-time PCR (qRT-PCR) and bisulfite sequencing PCR (BSP) following 24â¯h of LPS treatment. Inhibition of DNA methylation significantly enhanced LPS-induced IL-6 and IL-8 mRNA expression. LPS increased IL-6 and IL-8 mRNA expression, and decreased methylation levels of specific CpG sites at the IL-6 promoter (at â366 and â660) and the IL-8 promoter (at â120 and â48) after 24â¯h. Furthermore, LPS treatment for 24â¯h significantly increased DNMT1, DNMT3A, DNMT3B, and MeCP2 mRNA expression. Our results indicate that treating bovine endometrial cells with LPS induces the expression of IL-6 and IL-8 mRNA regulated by IL-6 and IL-8 promoter methylation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 677, 30 November 2018, Pages 266-272
Journal: Gene - Volume 677, 30 November 2018, Pages 266-272
نویسندگان
Jun Wang, Xiaoxiao Yan, Lucky T. Nesengani, He Ding, Lianyu Yang, Wenfa Lu,