کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644514 | 1569761 | 2018 | 49 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome-wide transcriptome profiling provides insights into panicle development of rice (Oryza sativa L.)
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کلمات کلیدی
QTLfragments per kilobase of transcript per million fragments mappedPentatricopeptide repeat proteinNILCluster of Orthologous Groups of proteinsFPKMPPRDEGsqRT-PCRRNA-seqCyPFDRSBPCoGRice - برنجTranscriptome - ترانسکریپتومKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوcytochrome P450 monooxygenase - سیتوکروم P450 monooxygenasequantitative trait locus - علامت کمی صفتfalse discovery rate - میزان کشف کاذبnear isogenic lines - نزدیک خطوط ایزوگنیکPanicle - هراسGene ontology - هستیشناسی ژنیnon-redundant protein database - پایگاه داده پروتئین غیر انحصاریDifferentially expressed genes - ژن های متفاوت بیان شده است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Panicle architecture is an important component of agronomic trait in rice, which is also a key ingredient that could influence yield and quality of rice. In the panicle growth and development process, there are a series of complicated molecular and cellular events which are regulated by many interlinking genes. In this study, to explore the potential mechanism and identify genes and pathways involved in the formation of rice panicle, we compared the transcriptional profile of rice panicles (NIL-GW8 and NIL-gw8Amol) at three different stages of panicle development: In5 (formation of higher-order branches), In6 (differentiation of glumes) and In7 (differentiation of floral organs). A range of 40.5 to 54.1 million clean reads was aligned to 31,209 genes in our RNA-Seq analysis. In addition, we investigated transcriptomic changes between the two rice lines during different stages. A total of 726, 1121 and 2584 differentially expressed genes (DEGs) were identified at stages 1, 2 and 3, respectively. Based on an impact analysis of the DEGs, we hypothesize that MADS-box gene family, cytochrome P450 (CYP) and pentatricopeptide repeat (PPR) protein and various transcription factors may be involved in regulation of panicle development. Further, we also explored the functional properties of DEGs by gene ontology analysis, and the results showed that different numbers of DEGs genes were associated with 53 GO groups. In KEGG pathway enrichment analysis, many DEGs related to biosynthesis of secondary metabolites and plant hormone signal transduction, suggesting their important roles during panicle development. This study provides the first examination of changes in gene expression between different panicle development stages in rice. Our results of transcriptomic characterization provide important information to elucidate the complex molecular and cellular events about the panicle formation in rice or other cereal crops. Also, the findings will be helpful for the further identification of the genes related to panicle development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 675, 30 October 2018, Pages 285-300
Journal: Gene - Volume 675, 30 October 2018, Pages 285-300
نویسندگان
Shanwen Ke, Xin-Jiang Liu, Xin Luan, Weifeng Yang, Haitao Zhu, Guifu Liu, Guiquan Zhang, Shaokui Wang,