کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644651 | 1569764 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
KatG plays an important role in Aeromonas hydrophila survival in fish macrophages and escape for further infection
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کلمات کلیدی
A. hydrophilaFCSRNSNBTTSBFrancisella tularensisRT-qPCRMICAeromonas hydrophilaORFTSAMBTE. coliPBSF. tularensisC. jejuni− - -DMSO - DMSOMOI - MERNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهROS - ROSnitroblue tetrazolium - tetrazolium nitroblueTryptic Soy Agar - Tryptic من آگار هستمHydrogen peroxide - آب اکسیژنهStreptomycin - استروتومایسینEscherichia coli - اشریشیا کُلیIntracellular survival - بقای درون سلولیMAS - بیشترTetracycline - تتراسایکلینBase pair(s) - جفت پایه (ها)Minimum inhibitory concentration - حداقل غلظت مهاریdeletion - حذفescape - در رفتنdimethyl sulphoxide - دی متیل سولفوکسیدfoetal calf serum - سرم گوساله جنینtrypticase soy broth - سیب سویاopen reading frame - قاب خواندن بازLuria-Bertani (medium) - لوریا بارتانی (متوسط)Mycobacterium tuberculosis - مایکوباکتریوم توبرکلوزیسPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریH2O2 - هیدروژن پراکسیدReverse Transcription-quantitative Polymerase Chain Reaction - واکنش زنجیره ای پلیمراز کمی رونویسی معکوسPlasmid - پلاسمیدmultiplicity of infection - چندین عفونتoptical density - چگالی نوریCampylobacter jejuni - کامپیلوباکتر ژژونیkatG - کتChloramphenicol - کلرامفنیکلreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The success of the pathogenic bacteria is partly attributable to their ability to thwart host innate immune responses, which includes resisting the antimicrobial functions of macrophages. And reactive oxygen species (ROS) is one of the most effective antimicrobial components of macrophages to kill invading bacteria. Our previous studies found that Aeromonas hydrophila can survive in fish macrophages, which suggested that this bacterium might take fish macrophages as their shelters to resist drug killings and other immune damage. But how A. hydrophila survive in host macrophages remains unknown. Since KatG has been reported to have not only catalase activity but also peroxidase and peroxynitritase activity, the amino acid sequence and protein structure of KatG was analyzed in this study, the function of KatG in A. hydrophila survival in and escape from host macrophages was also carried out. The bioinformatics analysis displayed that KatG of A. hydrophila B11 showed >93% homologous to that of KatG in other Aeromonas. KatG of A. hydrophila was stable silenced by shRNA and RT-qPCR confirmed the expression of KatG in KatG-RNAi was significantly reduced. The survival rate of intracellular KatG-RNAi decreased by 80% compared to that of the wild type strain B11, while the intracellular ROS level of the macrophages that phagocytosed KatG-RNAi increased 65.9% when compared to that of the macrophages phagocytosed wild-type strain. The immune escape rate of A. hydrophila decreased by 85% when the expression of KatG was inhibited. These results indicated that (1) The amino acid sequence and protein structure of KatG of A. hydrophila is conserved; (2) KatG helped A. hydrophila to survive in fish macrophages by eliminating the harm of intracellular H2O2 and inhibiting intracellular ROS levels increased; (3) A small portion of intracellular A. hydrophila could escape from host macrophages for further infection, in this process KatG also played important role.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 672, 25 September 2018, Pages 156-164
Journal: Gene - Volume 672, 25 September 2018, Pages 156-164
نویسندگان
Meimei Zhang, Qingpi Yan, Leilei Mao, Suyun Wang, Lixing Huang, Xiaojin Xu, Yingxue Qin,