کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645005 | 1569773 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Down-regulated let-7b-5p represses glycolysis metabolism by targeting AURKB in asthenozoospermia
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کلمات کلیدی
qRT-PCRECARTLDATaqMan low density arrayidiopathic male infertilityasthenozoospermia - آستنوسوپرمیMicroRNA - میکرو RNA extracellular acidification rate - نرخ اسیدی شدن خارج سلولیQuantitative reverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوس کمیGlycolysis - گلیکولیز یا قندکافت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Down-regulated let-7b-5p represses glycolysis metabolism by targeting AURKB in asthenozoospermia Down-regulated let-7b-5p represses glycolysis metabolism by targeting AURKB in asthenozoospermia](/preview/png/8645005.png)
چکیده انگلیسی
Glycolysis, through anaerobic respiration, can supply energy for human sperm motility. MicroRNAs (miRNAs) could participate in the glycolytic pathway through regulating target genes. To investigate the potential role of glycolysis-related miRNAs in asthenozoospermia, TaqMan Low Density Array (TLDA) was used to screen potentially functional miRNAs, and seven glycolysis-related miRNAs were isolated to be related to asthenozoospermia. After qRT-PCR validation, only one seminal plasma miRNA, let-7b-5p, was found significantly decreased in severe asthenozoospermia cases compared with healthy controls. To further understand whether let-7b-5p is involved in asthenozoospermia by regulating the glycolytic pathway, we carried out gain-and-loss function study of let-7b-5p in GC-2 cells and detected the glycolytic activities. Our results showed that knocking down let-7b-5p could inhibit glycolytic activities. Besides, we also found overexpressed Aurkb (a target gene of let-7b-5p) could recapitulate the effects of knocking down let-7b-5p. Our findings indicated that low expression of let-7b-5p could repress glycolysis in asthenozoospermia by targeting AURKB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 663, 15 July 2018, Pages 83-87
Journal: Gene - Volume 663, 15 July 2018, Pages 83-87
نویسندگان
Ran Zhou, Yan Zhang, Guizhen Du, Li Han, Sinian Zheng, Jian Liang, Xiaomin Huang, Yufeng Qin, Wei Wu, Minjian Chen, Di Wu, Ling Song, Guangbo Fu, Shuyan Lv, Yankai Xia, Chuncheng Lu, Xinru Wang,