کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645156 | 1569777 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
SP1-mediated downregulation of ADAMTS3 gene expression in osteosarcoma models
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ECMMG-63USFAP1ADAMTSSaOS-2BSPvWFEDSC/EBPα - C / EBPαSp1 - SP1ALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییSTAT - آمارOsteosarcoma - استئوسارکوماOsteocalcin - استئوکلسین a disintegrin and metalloproteinase with thrombospondin motifs - تخریب و متالوپروتئیناز با ترومبوسپوندین موتیفTranscriptional regulation - تنظیم ترانزیتیEhlers–Danlos syndrome - سندرم Ehlers-Danlosbone sialoprotein - سیلوپروتئین استخوانVon Willebrand factor - عامل فون ویلبراندUpstream Stimulatory Factor 1 - فاکتور رشد رو به بالا 1Extracellular matrix - ماتریکس خارج سلولیSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیactivator protein 1 - پروتئین فعال کننده 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
ADAMTS3 is a member of procollagen N-proteinase subfamily of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) gene family. It has an important function in the procollagen maturation process. The removal of N-peptidases is required for the accurate processing of fibrillar collagens. Otherwise, several disorders can occur that is related with the collagenous tissues. ADAMTS3 mainly maturates type II collagen molecule which is the main component of the bone and cartilage. There are several expression studies about ADAMTS3 gene however its transcriptional regulation has not been lightened up, yet. Here we first time cloned and functionally analyzed the promoter region of ADAMTS3 gene, approximately 1380â¯bp upstream of the transcription start site. Transient transfection experiments showed that all truncated promoter constructs are active and 171â¯bp fragment is sufficient to activate gene expression in both Saos-2 and MG63 cells. In silico analysis showed that ADAMTS3 has a TATA-less promoter and contains several SP1/GC box binding motifs and a CpG island. Therefore we mainly investigated the SP1 dependent regulation of ADAMTS3 promoter. SP1 downregulated ADAMTS3 transcriptional activity. As consistent with the transcriptional activity, mRNA, and protein expression levels were also decreased by SP1. On the other hand, functional binding of the SP1 on multiple regions of ADAMTS3 promoter was confirmed by EMSA studies. As ADAMTS3 is responsible for the collagen maturation and biosynthesis, further we investigated the effect of SP1 on type I-II and III collagen gene expressions. We point out that SP1 increased type II and III collagen expression and in contrast decreased type I collagen expression levels in Saos-2 cells. mRNA expression level was decreased for all collagen types in MG63 model. Decrease in the type II collagen expression was also demonstrated at the protein level by SP1. Collectively these results provide first findings for the SP1-related transcriptional regulation of ADAMTS3 and collagen genes in osteosarcoma cell lines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 659, 15 June 2018, Pages 1-10
Journal: Gene - Volume 659, 15 June 2018, Pages 1-10
نویسندگان
A.TuÄÅen Aydemir, Meltem Alper, Feray Kockar,