کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645184 | 1569777 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
KLF15 promotes transcription of KLF3 gene in bovine adipocytes
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کلمات کلیدی
EAMCKLF3KLF15Krüppel-like factorsKLFRT-PCRCtBPPPARγGLUT4qPCRMOI - MEsiRNA - siRNAElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزchromatin immunoprecipitation - ایمن سازی کروماتینElectrophoretic Mobility Shift - تغییر حرکت الکترو فوریتیreverse transcription PCR - رونویسی معکوس PCREMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکIMF - صندوق بین المللی پولIntramuscular fat content - محتوای چربی داخل عضلانیquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیGlucose transport protein - پروتئین حمل گلوکزPromoter - پروموترmultiplicity of infection - چندین عفونتCHiP - چیپperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Krüppel-like factors (KLF) family plays an important role in adipogenesis, which is subject to internal hierarchical regulation. KLF3 is a member of KLF family, mainly responsible for adipocyte differentiation and fat deposition. However, the transcriptional regulation of bovine KLF3 gene and its relationship with KLF15 gene remains unclear during bovine adipogenesis. Here, we report that the expression pattern of KLF3 and KLF15 genes during bovine adipogenesis, when KLF15 gene was overexpressed through adenoviral vector (Ad-KLF15) in bovine adipocytes the expression level of KLF3 gene was increased, similarly when KLF15 was down regulated through siRNA the expression level of KLF3 was also reduced. To explore the transcriptional regulation of bovine KLF3 gene and its relationship with KLF15, serial deletion constructs of the 5â²flanking region of bovine KLF3gene revealed through dual-luciferase reporter assay that the core promoter is located in â264 to â76 regions. The most proximal GGGG element in the promoter of the bovine KLF3 gene (located in â264 to â76 regions) is required for promotion by KLF15. Electrophoretic mobility shift (EMSA) and chromatin immunoprecipitation (ChIP) assays further confirmed that KLF15 gene binds to the KLF3 gene core promoter region in bovine adipocytes. These findings conclude that KLF15 promotes the transcriptional activity of KLF3 in bovine adipocytes. This mechanism to provides a new direction for further study of adipogenesis by internal regulation of members within KLF family.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 659, 15 June 2018, Pages 77-83
Journal: Gene - Volume 659, 15 June 2018, Pages 77-83
نویسندگان
Hongfang Guo, Rajwali Khan, Sayed Haidar Abbas Raza, Yue Ning, Dawei Wei, Sen Wu, Seyed Mahdi Hosseini, Irfan Ullah, Matthew D. Garcia, Linsen Zan,