کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645414 | 1569785 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of calcium-dependent protein kinase (CPK) 2 gene from oilseed rape (Brassica napus L.) in regulating reactive oxygen species signaling and cell death control
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کلمات کلیدی
MDAVPEGFPRT-PCRBrassica napusqRT-PCRBiFCSAGVacuolar processing enzymePCDRboh - R ب OHROS - ROSquantitative RT-PCR - RT-PCR کمیrespiratory burst oxidase homolog - اکسیداز انفجار تنفسیbimolecular fluorescence complementation - تکمیل فلورسانس دو طرفه حرکتیmalondialdehyde - مالون دی آلدهیدMetacaspase - متاکاسپازpathogenesis-related - مرتبط با پاتوژنزProgrammed cell death - مرگ برنامهریزی شده یاختهCell death - مرگ سلولی reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسGreen fluorescence protein - پروتئین فلورسانس سبزCalcium-dependent protein kinase - پروتئین کیناز وابسته به کلسیمSenescence-associated gene - ژن وابسته به سن زبانیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Calcium-dependent protein kinases (CPKs), being Ser/Thr protein kinases found only in plants and some protozoans are calcium sensors that regulate diverse biological processes. However, the function and mode of CPKs in oilseed rape (Brassica napus) remain elusive. In this study, we identified CPK2 from oilseed rape as a novel regulator of reactive oxygen species (ROS) and cell death. BnaCPK2 was identified to be located at the endoplasmic reticulum membrane. Expression of BnaCPK2 was induced during Bax-induced cell death. Overexpression of the constitutively active form of BnaCPK2 led to significantly more accumulation of ROS and cell death than the full-length CPK2, which is supported by various measurements of physiological data. In addition, a quantitative RT-PCR survey revealed that the expression levels of a few marker genes are significantly changed as a result of CPK2 expression. Mating-based split ubiquitin system (mbSUS) and bimolecular fluorescence complementation (BiFC) were used to screen and confirm the BnaCPK2 interacting proteins. We identified and confirmed that CPK2 interacted with NADPH oxidase-like respiratory burst oxidase homolog D (RbohD), but not with RbohF. Based on its function and interacting partners, we propose that BnaCPK2 plays an important role in ROS and cell death control through interacting with RbohD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 651, 20 April 2018, Pages 49-56
Journal: Gene - Volume 651, 20 April 2018, Pages 49-56
نویسندگان
Weihuan Wang, Hanfeng Zhang, Xiangyan Wei, Liu Yang, Bo Yang, Li Zhang, Jing Li, Yuan-Qing Jiang,