کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645577 | 1569790 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of the AQP members involved in abiotic stress responses from Arabidopsis
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کلمات کلیدی
small basic intrinsic proteinsMDAAQPsqRT-PCRNIPSAquaporinspIPS - PIPSArabidopsis - آرابیدوپسیس یا رشادیaquaporin - آکواپورینAbiotic stresses - استرس های بی روحSips - سیر می شودFunctional identification - شناسایی عملکردmalondialdehyde - مالون دی آلدهیدwild type - نوع وحشیTIPS - نکاتquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیtonoplast intrinsic proteins - پروتئین های ذاتی تنوپلاستplasma membrane intrinsic proteins - پروتئین های ذاتی غشاء پلاسما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aquaporins (AQPs) constitute a highly diverse family of water channel proteins that play crucial biological functions in plant growth and development and stress physiology. In Arabidopsis, 35 AQPs are classified into four subfamilies (PIPs, TIPs, NIPs and SIPs). However, knowledge about the roles of different subfamily AQPs remains limited. Here, we explored the chromosomal location, gene structure and expression patterns of all AQPs in different tissues or under different abiotic stresses based on available microarray data. Tissue expression analysis showed that different AQPs had various expression patterns in tissues (root, leaf, flower and seed). Expression profiles under stress conditions revealed that most AQPs were responsive to osmotic, salt and drought stresses. Phenotypic and physiological identification showed that Tip2;2 loss-of-function mutant exhibited less sensitive to abiotic stresses (mannitol, NaCl and PEG) compared with wild-type, as evident by analysis of germination rate, root growth, survival rate, ion leakage, malondialdehyde (MDA) and proline contents. Mutant of TIP2;2 modulated the transcript levels of SOS1, SOS2, SOS3, DREB1A, DREB2A and P5CS1, under abiotic stress conditions. This study provides a basis for further functional identification of stress-related candidate AQPs in Arabidopsis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 646, 10 March 2018, Pages 64-73
Journal: Gene - Volume 646, 10 March 2018, Pages 64-73
نویسندگان
Zhi-Juan Feng, Sheng-Chun Xu, Na Liu, Gu-Wen Zhang, Qi-Zan Hu, Zhao-Shi Xu, Ya-Ming Gong,