کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645779 | 1569792 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
AJUBA increases the cisplatin resistance through hippo pathway in cervical cancer
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کلمات کلیدی
ESCCHippo signaling pathwayAJUBALats1/2YAP - ایجادImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیEMT - تکنسین فوریتهای پزشکیCervical cancer - سرطان دهانه رحمlarge tumor suppressor - سرکوب کننده تومور بزرگCisplatin resistance - مقاومت سیس پلاتینyes-associated protein - پروتئین مرتبط با بلهEsophageal squamous cell carcinoma - کارسینوم سلول سنگفرشی مریepithelial-to-mesenchymal transition - گذار اپیتلیال به مزانشیمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Though LIM-domain protein AJUBA was identified as a putative oncogene, the function and underlying mechanisms of AJUBA in cervical cancer remain largely unknown. Firstly, AJUBA expression was detected via real-time quantitative PCR in patients' samples. Furthermore, Hela and Siha cells were transfected with AJUBA-overexpressing plasmids, and then exposed to cisplatin, the apoptosis was measured by cytometry assay. In addition, the expression of YAP and TAZ was disclosed through western blot assay. Our results revealed that AJUBA expression was significantly higher in the cervical cancer patients resistant to cisplatin treatment compared with cervical cancer patients sensitive to cisplatin treatment. In addition, overall survival time was significantly shorter in the cervical cancer patients with high AJUBA expression compare with those with low AJUBA expression using kaplan-meier analysis. Hela and Siha cells transfected with AJUBA-expressing plasmids exposed to cisplatin treatment had higher survival rate compared with the cells transfected with empty vector control. Mechanistic studies revealed the AJUBA upregulated the downstream targets YAP and TAZ. These results suggest that high AJUBA level enhances cervical cancer cells drug resistance to cisplatin, also associates with decreased patient survival times.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 644, 20 February 2018, Pages 148-154
Journal: Gene - Volume 644, 20 February 2018, Pages 148-154
نویسندگان
Lihong Bi, Feng Ma, Rui Tian, Yanli Zhou, Weiguang Lan, Quanmao Song, Xiankui Cheng,