کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645809 | 1569793 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced chondrogenesis of human bone marrow mesenchymal Stem Cell (BMSC) on nanofiber-based polyethersulfone (PES) scaffold
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کلمات کلیدی
GAPDHCOL1A1BMSCCol2a1collagen type XMSCFGFCol10a1PESTCPSTGF-βFBSDMEMPBSDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoOsteoarthritis - استئوآرتریت(آرتروز)transforming growth factor-β - تبدیل فاکتور رشد βfetal bovine serum - سرم جنین گاوBone marrow mesenchymal stem cell - سلول بنیادی مزانشیمی مغز استخوانMesenchymal stem cells - سلول های بنیادی مزانشیمیfibroblast growth factor - فاکتور رشد فیبروبلاستphosphate-buffered saline (PBS) - فسفات بافر شور (PBS)SEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیBMP - مدیریت فرایند کسب و کارscanning electron microscope - میکروسکوپ الکترونی اسکنbone morphogenetic proteins - پروتئین های مورفوژنیک استخوانpolyethersulfone - پلی اترسولفونTissue culture polystyrene - پلی استایرن کشت بافتCollagen type I - کلاژن نوع Icollagen type II - کلاژن نوع IIChondrocytes - کندروسیتglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mesenchymal stem cells (MSC) from bone marrow hold great potential as a cell source for cartilage repair. The objective of our study was differentiation of MSC toward chondrocyte by using Nanofiber-based polyethersulfone (PES) scaffold and also enhanced chondrogenic differentiation of BMSC in vitro. MSCs were harvested from bone marrow of human and PES scaffold was fabricated via Electrospinning. The isolated cells were cultured on the PES scaffold and scaffold free method. After 21Â days, Real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. Also, in order to confirm our results, we have done immunocytochemistry and SEM imaging. Flowcytometry confirmed the nature of the isolated adherent cells. Immunocytochemistry and SEM imaging confirmed the differentiation of MSC toward chondrocyte. Also, real time PCR showed a significant increased gene expression of collagen type II and aggrecan on the PES scaffold method when compared to the mRNA levels measured in scaffold free method. Down regulation of Collagen type I was observed in PES scaffold compared to scaffold free at day 21. Also, both methods showed a similar pattern of expression of SOX9. Our results showed that PES scaffold maintains BMSC proliferation and differentiation, and can significantly enhance chondrogenic differentiation of BMSC. PES scaffold seeded BMSC showed the highest capacity for differentiation into chondrocyte-like cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 643, 15 February 2018, Pages 98-106
Journal: Gene - Volume 643, 15 February 2018, Pages 98-106
نویسندگان
Hossein Mahboudi, Bahram Kazemi, Masoud Soleimani, Hana Hanaee-Ahvaz, Hossein Ghanbarian, Mojgan Bandehpour, Seyed Ehsan Enderami, Mousa Kehtari, Ghasem Barati,