کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645995 | 1569795 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cidec differentially regulates lipid deposition and secretion through two tissue-specific isoforms
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کلمات کلیدی
DMEMqPCRVLDLCIDECDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccostandard deviation - انحراف معیارIsoform - ایسوفورlipid secretion - ترشح چربیtriglyceride - تریگلیسریدPig - خوکLipid deposition - رسوب لیپیدLipid droplets - قطرات لیپیدvery low-density lipoprotein - لیپوپروتئین بسیار کم چگالیquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lipid metabolism has important roles in animal growth, development, and reproduction. As a regulator of lipid metabolism, CIDEc promotes unilocular development of lipid droplets and stimulates intracellular lipid deposition, and has two isoforms, CIDEc-l and CIDEc-s. CIDEc-l has ten more N-terminal amino acids than CIDEc-s. However, the functions of two isoforms are largely unknown. In this study, the expression profiles of two isoforms in Bama pigs differed, with cidec-l dominant in the liver and small intestine, and cidec-s dominant in muscle and adipose tissue. Fasting and consuming a high-fat diet resulted in changes in the expression of the two isoforms that were closely related to changes in blood and muscle triglyceride (TG) concentrations. Comparison of gene expression and TG concentration suggested that CIDEc-l accelerated lipid secretion and that CIDEc-s promoted lipid deposition, implying that the two isoforms had different functions. Study In vitro confirmed that CIDEc-s stimulated lipid deposition in C2C12 muscle cells and CIDEc-l promoted lipid secretion in HepG2 liver cells. The results showed that two tissue-specific CIDEc isoforms had different roles in lipid deposition and secretion. They may be potential targets for regulation of fat content.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 641, 30 January 2018, Pages 265-271
Journal: Gene - Volume 641, 30 January 2018, Pages 265-271
نویسندگان
Yixing Li, Huifang Kang, Yi Chu, Yi Jin, Lifang Zhang, Ranran Yang, Zhiwang Zhang, Shuhong Zhao, Lei Zhou,