کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8646403 1570116 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identifying protective host gene expression signatures within the spleen during West Nile virus infection in the collaborative cross model
ترجمه فارسی عنوان
شناسایی علامت بیان ژن میزبان محافظ در طحال در طی عفونت ویروس غرب نیل در مدل متقابل مشترک
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی
Flaviviruses are hematophagous arthropod-viruses that pose global challenges to human health. Like Zika virus, West Nile Virus (WNV) is a flavivirus for which no approved vaccine exists [1]. The role host genetics play in early detection and response to WNV still remains largely unexplained. In order to capture the impact of genetic variation on innate immune responses, we studied gene expression following WNV infection using the collaborative cross (CC). The CC is a mouse genetics resource composed of hundreds of independently bred, octo-parental recombinant inbred mouse lines [2]. To accurately capture the host immune gene expression signatures of West Nile infection, we used the nanostring platform to evaluate expression in spleen tissue isolated from CC mice infected with WNV over a time course of 4, 7, and 12 days' post-infection [3]. Nanostring is a non-amplification based digital method to quantitate gene expression that uses color-coded molecular barcodes to detect hundreds of transcripts in a sample. Using this approach, we identified unique gene signatures in spleen tissue at days 4, 7, and 12 following WNV infection, which delineated distinct differences between asymptomatic and symptomatic CC lines. We also identified novel immune genes. Data was deposited into the Gene Expression Omnibus under accession GSE86000.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics Data - Volume 10, December 2016, Pages 114-117
نویسندگان
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