کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8648257 | 1570442 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid increase of near atomic resolution virus capsid structures determined by cryo-electron microscopy
ترجمه فارسی عنوان
افزایش سریع ساختارهای کپسید ویروس با وضوح نزدیک به اتمی که توسط میکروسکوپ الکترونی کریو تعیین شده است
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
چکیده انگلیسی
The recent technological advances in electron microscopes, detectors, as well as image processing and reconstruction software have brought single particle cryo-electron microscopy (cryo-EM) into prominence for determining structures of bio-molecules at near atomic resolution. This has been particularly true for virus capsids, ribosomes, and other large assemblies, which have been the ideal specimens for structural studies by cryo-EM approaches. An analysis of time series metadata of virus structures on the methods of structure determination, resolution of the structures, and size of the virus particles revealed a rapid increase in the virus structures determined by cryo-EM at near atomic resolution since 2010. In addition, the data highlight the median resolution (â¼3.0â¯Ã
) and size (â¼310.0â¯Ã
in diameter) of the virus particles determined by X-ray crystallography while no such limits exist for cryo-EM structures, which have a median diameter of 508â¯Ã
. Notably, cryo-EM virus structures in the last four years have a median resolution of 3.9â¯Ã
. Taken together with minimal sample requirements, not needing diffraction quality crystals, and being able to achieve similar resolutions of the crystal structures makes cryo-EM the method of choice for current and future virus capsid structure determinations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 201, Issue 1, January 2018, Pages 1-4
Journal: Journal of Structural Biology - Volume 201, Issue 1, January 2018, Pages 1-4
نویسندگان
Phuong T. Ho, Vijay S. Reddy,