کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8649160 | 1570836 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression analysis of defense related genes in wheat and maize against Bipolaris sorokiniana
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression analysis of defense related genes in wheat and maize against Bipolaris sorokiniana Expression analysis of defense related genes in wheat and maize against Bipolaris sorokiniana](/preview/png/8649160.png)
چکیده انگلیسی
Wheat (Triticum aestivum L.) and maize (Zea mays L.) are important cereal crops grown worldwide. In Pakistan, various varieties of wheat and maize are grown and a number of biotic and abiotic factors influence its production. These are mainly used as a food source and raw material for animal feed. Bipolaris sorokiniana is a hemibiotrophic phytopathogenic fungus that affects various grass species and causes number of diseases on small grains. An investigation was undertaken to evaluate the comparative expression of pathogenesis-related protein genes in wheat and maize under B. sorokiniana stress. After stress, these crops showed disease resistance by improving biochemical parameters like proline, protein, sugar and chlorophyll content. Morpho-physiological parameters including shoot length, root length, relative water content and fresh as well as dry weight were affected by fungal stress. Expression analysis of PR-proteins in stressed plants of wheat confirmed their role in plant defence. PR-1, Thaumatin-like protein (TLP), and β 1,3 glucanase were highly expressed under stress in wheat variety (RWP13), it suggests the role of these genes in the prescribed variety. These genes did not show their expression in susceptible wheat variety (SWG13). Zm PR-5 and Zm 1,3 β-glucanase were highly expressed in maize variety (RMN27) after stress which suggests their role in defence mechanism of this variety. While on the other hand, no significant expression of these genes was observed in susceptible maize variety (SMIG). In future, these genes can be over-expressed in transgenic plants to make them resistant against a number of biotic and abiotic factors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 103, August 2018, Pages 36-46
Journal: Physiological and Molecular Plant Pathology - Volume 103, August 2018, Pages 36-46
نویسندگان
Hakim Manghwar, Amjad Hussain, Abid Ullah, Summia Gul, Muhammad Shaban, Aamir Hamid Khan, Musrat Ali, Syed Gul Abbas Shah Sani, Hassan Javed Chaudhary, Muhammad Farooq Hussain Munis,