کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8650262 | 1571124 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute aerobic exercise induces a preferential mobilisation of plasmacytoid dendritic cells into the peripheral blood in man
ترجمه فارسی عنوان
ورزش هوازی حاکی از بسیج ترجیحی سلول های دندریتیک پلاسمایی سوزنی به خون محیطی در انسان است
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کلمات کلیدی
سلولهای دندریتیک، میلوئید، پلاسماسوئید، ورزش، انسان،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
فیزیولوژی
چکیده انگلیسی
Dendritic cells (DCs) are important sentinel cells of the immune system responsible for presenting antigen to T cells. Exercise is known to cause an acute and transient increase in the frequency of DCs in the bloodstream in humans, yet there are contradictory findings in the literature regarding the phenotypic composition of DCs mobilised during exercise, which may have implications for immune regulation and health. Accordingly, we sought to investigate the composition of DC sub-populations mobilised in response to acute aerobic exercise. Nine healthy males (age, 21.9â¯Â±â¯3.6â¯years; height, 177.8â¯Â±â¯5.4â¯cm; body mass, 78.9â¯Â±â¯10.8â¯kg; body mass index, 24.9â¯Â±â¯3.3â¯kg·m2; VÌO2 MAX, 41.5â¯Â±â¯5.1â¯mL·kg·minâ1) cycled for 20â¯min at 80% VÌO2 MAX. Blood was sampled at baseline, during the final minute of exercise and 30â¯min later. Using flow cytometry, total DCs were defined as Lineageâ (CD3, CD19, CD20, CD14, CD56) HLA-DR+ and subsequently identified as plasmacytoid DCs (CD303+) and myeloid DCs (CD303â). Myeloid DCs were analysed for expression of CD1c and CD141 to yield four sub-populations; CD1câCD141+; CD1c+CD141+; CD1c+CD141â and CD1câCD141â. Expression of CD205 was also analysed on all DC sub-populations to identify DCs capable of recognising apoptotic and necrotic cells. Total DCs increased by 150% during exercise (F(1,10)â¯=â¯60; pâ¯<â¯0.05, η2â¯=â¯0.9). Plasmacytoid DCs mobilised to a greater magnitude than myeloid DCs (195â¯Â±â¯131% vs. 131â¯Â±â¯100%; pâ¯<â¯0.05). Among myeloid DCs, CD1câCD141â cells showed the largest exercise-induced mobilisation (167â¯Â±â¯122%), with a stepwise pattern observed among the remaining sub-populations: CD1c+CD141â (79â¯Â±â¯50%), followed by CD1c+CD141+ (44â¯Â±â¯41%), with the smallest response shown by CD1câCD141+ cells (23â¯Â±â¯54%) (pâ¯<â¯0.05). Among myeloid DCs, CD205â cells were the most exercise responsive. All DC subsets returned to resting levels within 30â¯min of exercise cessation. These results show that there is a preferential mobilisation of plasmacytoid DCs during exercise. Given the functional repertoire of plasmacytoid DCs, which includes the production of interferons against viral and bacterial pathogens, these findings indicate that exercise may augment immune-surveillance by preferentially mobilising effector cells; these findings have general implications for the promotion of exercise for health, and specifically for the optimisation of DC harvest for cancer immunotherapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiology & Behavior - Volume 194, 1 October 2018, Pages 191-198
Journal: Physiology & Behavior - Volume 194, 1 October 2018, Pages 191-198
نویسندگان
Frankie F. Brown, John P. Campbell, Alex J. Wadley, James P. Fisher, Sarah Aldred, James E. Turner,