کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8666 602 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The recruitment of two consecutive and different waves of host stem/progenitor cells during the development of tissue-engineered bone in a murine model
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The recruitment of two consecutive and different waves of host stem/progenitor cells during the development of tissue-engineered bone in a murine model
چکیده انگلیسی

Angiogenesis plays a central role in bone regeneration, not only for the transport of nutrients, but also for locally directing skeletal stem/progenitor cells. Following ectopic implantation of porous ceramic cubes seeded with mouse GFP-labeled mesenchymal stem cells (MSC) into syngenic mice, we investigated the cascade of events leading to bone formation. Implants harvested at different times were enzymatically digested to generate single-cell suspensions. Recovered cells were sorted to separate GFP + implanted MSC and host recruited GFP- cells. We isolated and characterized two different waves of cells, migrating from the host to the MSC-seeded ceramic. The first migrated cell population, recovered 7 days after implantation, was enriched in CD31 + endothelial progenitors, while the second one, recruited at day 11, was enriched in CD146 + pericyte-like cells. Both populations were not recruited into the scaffold following implantation of a non-MSC seeded ceramic. Pericyte-like cell mobilization was dependent on the first migrated endothelial cell population. Pericyte-like cells retained properties distinctive of stem cells, such as capacity of performing a high number of in vitro cell divisions and showed an osteogenic potential. Studies on the cross talk between implanted exogenous MSC and resident stem/progenitor cells could open new perspectives for future clinical applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 8, March 2010, Pages 2121–2129
نویسندگان
, , , , ,