Validation of Biofilm Assays to Assess Antibiofilm Efficacy in Instrumented Root Canals after Syringe Irrigation and Sonic Agitation

This study highlighted the maximum reduction of microbes after instrumentation-syringe irrigation. Although supplementary sonic agitation reduced the root canal biofilm further, it did not completely eliminate the biofilm from a single root canal model. The merits of combining microbiological and molecular quantification methods with CLSM for the comprehensive assessment of antibiofilm efficacy in root canals were emphasized.