کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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871996 | 910217 | 2015 | 6 صفحه PDF | دانلود رایگان |
The validation of the AFM method for elastic modulus E measurement in soft materials (E <5 MPa) is still missing. The interest of measurements in materials with E <5 MPa is mainly biological, including soft tissues and single cells. For the diagnosis of malignant human tumors, a change in cell elasticity, within tissues, has recently been recognized as a marker of metastatic potential. To measure a cell elasticity difference, reproducible E measurements in biological samples are needed. In this work a robust method for a metrological validation of E measurements in the range 500–5000 kPa was developed, based on the realization of thick E standard samples and on the study of the interactions between the measurement process and the sample at micro- and nano-scale. E measurement reproducibility limit of 4% has been reached. This allows designing a very sensitive and reproducible measurement of E in biological samples representing thus a powerful diagnostic tool for cancer detection.
Journal: Journal of Biomechanics - Volume 48, Issue 6, 13 April 2015, Pages 1099–1104