کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840764 | 1614697 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Allopregnanolone Effects on Transmission in the Brain Stem Solitary Tract Nucleus (NTS)
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کلمات کلیدی
HEPESEGTANucleus tractus solitariesaCSFTTXNTSevoked excitatory postsynaptic currenteEPSCAP5sIPSCsNBQX4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidd-2-amino-5-phosphonovalerate - d-2-amino-5-fosfonovalerateDMSO - DMSOgamma-aminobutyric acid - اسید گاما آمینو بوتیریکallopregnanolone - الوپرگنانولونALLO - بهtetrodotoxin - تترو دوتوکسین Spontaneous inhibitory postsynaptic currents - جریانهای پسینپتیک مهار کننده خود به خودیSolitary tract - دستگاه تناسلیDimethyl sulfoxide - دیمتیل سولفواکسیدartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
During pregnancy, the progesterone metabolite, allopregnanolone (ALLO), becomes elevated and has been associated with altered levels within the CNS and resulting changes in GABAA receptor function. Pregnant animals poorly compensate reflexes for a decrease in blood pressure during hemorrhage. Previous works suggested that ALLO decreases baroreflex responses by central actions, however, the underlying mechanisms are poorly understood. In this study, we tested ALLO actions on visceral afferent synaptic transmission at second-order neurons within medial portions of the nucleus tractus solitarius (NTS) using hindbrain slices from non-pregnant female rats. Solitary tract (ST) stimulation-evoked excitatory postsynaptic currents (ST-eEPSCs) in NTS neurons directly connected to vagal afferents within the ST. ST-eEPSCs were functionally identified as monosynaptic by the latency characteristics (low jitterâ¯=â¯standard deviation of latency, â¤200â¯Î¼s) to ST stimulation. Such second-order neurons all displayed spontaneous inhibitory postsynaptic currents (sIPSCs), and low micromolar concentrations of ALLO increased frequency and decay time. At submicromolar concentrations, ALLO induced a tonic, GABAergic inhibitory current and suppressed ST-eEPSCs' amplitude. While GABAA receptor antagonist, bicuculline, blocked all ALLO effects, gabazine only blocked sIPSC actions. In current-clamp mode, ALLO perfusion increased failure of ST stimulation to trigger action potentials in most neurons. Thus, our results indicate that ALLO acts to suppress visceral afferent ST synaptic transmission at first synapses by activating pharmacologically distinct GABAA subtypes at different concentration ranges. This ALLO-mediated attenuated visceral afferent signal integration in NTS may underlie reflex changes in blood pressure during gestation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 379, 21 May 2018, Pages 219-227
Journal: Neuroscience - Volume 379, 21 May 2018, Pages 219-227
نویسندگان
Sojin Kim, Sung-Moon Kim, Bermseok Oh, Jihoon Tak, Eunhee Yang, Young-Ho Jin,