کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840870 | 1614700 | 2018 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Histamine H3 Receptors Decrease Dopamine Release in the Ventral Striatum by Reducing the Activity of Striatal Cholinergic Interneurons
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FACSFSCVVTAaCSFGAPDHDTTCPMFBSStriatum - استریاتومOptogenetics - اپتوژنتیکcycle number - تعداد چرخهsubstantia nigra - توده سیاهtyrosine-hydroxylase - تیروزین هیدروکسیلازstandard error - خطای استانداردDopamine - دوپامینLight emitting diode - دیود ساطع نورdithiothreitol - دیتیوتریتولLED - رهبریfetal bovine serum - سرم جنین گاوcounts per minute - شمار در هر دقیقهfluorescence activated cell sorting - فلورسانس سلول فعال فعال سلولartificial cerebral spinal fluid - مایع مغزی نخاعی مغزیSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیventral tegmental area - ناحیه تگمنتوم شکمیCholinergic neurons - نورونهای کولینرژیکHistamine - هیستامینFast-scan cyclic voltammetry - ولتاژ سنجی چرخه سرعت اسکنChAT - چتcholine acetyltransferase - کولین استیل ترانسفرازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازH3 receptor - گیرنده H3
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Histamine H3 receptors are widely distributed Gi-coupled receptors whose activation reduces neuronal activity and inhibits release of numerous neurotransmitters. Although these receptors are abundantly expressed in the striatum, their modulatory role on activity-dependent dopamine release is not well understood. Here, we observed that histamine H3 receptor activation indirectly diminishes dopamine overflow in the ventral striatum by reducing cholinergic interneuron activity. Acute brain slices from C57BL/6 or channelrhodopsin-2-transfected DAT-cre mice were obtained, and dopamine transients evoked either electrically or optogenetically were measured by fast-scan cyclic voltammetry. The H3 agonist α-methylhistamine significantly reduced electrically- evoked dopamine overflow, an effect blocked by the nicotinic acetylcholine receptor antagonist dihydro-β-erythroidine, suggesting involvement of cholinergic interneurons. None of the drug treatments targeting H3 receptors affected optogenetically evoked dopamine overflow, indicating that direct H3-modulation of dopaminergic axons is unlikely. Next, we used qPCR and confirmed the expression of histamine H3 receptor mRNA in cholinergic interneurons, both in ventral and dorsal striatum. Activation of H3 receptors by α-methylhistamine reduced spontaneous firing of cholinergic interneurons in the ventral, but not in the dorsal striatum. Resting membrane potential and number of spontaneous action potentials in ventral-striatal cholinergic interneurons were significantly reduced by α-methylhistamine. Acetylcholine release from isolated striatal synaptosomes, however, was not altered by α-methylhistamine. Together, these results indicate that histamine H3 receptors are important modulators of dopamine release, specifically in the ventral striatum, and that they do so by decreasing the firing rate of cholinergic neurons and, consequently, reducing cholinergic tone on dopaminergic axons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 376, 15 April 2018, Pages 188-203
Journal: Neuroscience - Volume 376, 15 April 2018, Pages 188-203
نویسندگان
Rafael Koerich Varaschin, Guillaume Osterstock, Charles Ducrot, Sakari Leino, Marie-Josée Bourque, Marco A.M. Prado, Vania Ferreira Prado, Outi Salminen, Saara Rannanpää (née Nuutinen), Louis-Eric Trudeau,