کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8864 608 2010 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147
چکیده انگلیسی

We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 33, November 2010, Pages 8789–8801
نویسندگان
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