کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8883640 | 1625903 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomics investigation of thyroid hormone disruption in the olfactory system of the Rana [Lithobates] catesbeiana tadpole
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کلمات کلیدی
Endocrine disruption - اختلالات غدد درون ریزEstrogen - استروژنGene expression - بیان ژنPostembryonic development - توسعه پسامبوریونPharmaceuticals and personal care products - داروسازی و محصولات مراقبت شخصیMunicipal wastewater treatment - درمان فاضلاب شهریAmphibian - دوزیستانolfactory system - سیستم بویاییFrog - قورباغهTranscriptomics - متن ترانهThyroid hormone - هورمون تیروئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transcriptomics investigation of thyroid hormone disruption in the olfactory system of the Rana [Lithobates] catesbeiana tadpole Transcriptomics investigation of thyroid hormone disruption in the olfactory system of the Rana [Lithobates] catesbeiana tadpole](/preview/png/8883640.png)
چکیده انگلیسی
Thyroid hormones (THs) regulate vertebrate growth, development, and metabolism. Despite their importance, there is a need for effective detection of TH-disruption by endocrine disrupting chemicals (EDCs). The frog olfactory system substantially remodels during TH-dependent metamorphosis and the objective of the present study is to examine olfactory system gene expression for TH biomarkers that can evaluate the biological effects of complex mixtures such as municipal wastewater. We first examine classic TH-response gene transcripts using reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) in the olfactory epithelium (OE) and olfactory bulb (OB) of premetamorphic Rana (Lithobates) catesbeiana tadpoles after 48â¯h exposure to biologically-relevant concentrations of the THs, 3,5,3â²-triiodothyronine (T3) and L-thyroxine (T4), or 17-beta estradiol (E2); a hormone that can crosstalk with THs. As the OE was particularly sensitive to THs, further RNA-seq analysis found >30,000 TH-responsive contigs. In contrast, E2 affected 267 contigs of which only 57 overlapped with THs suggesting that E2 has limited effect on the OE at this developmental phase. Gene ontology enrichment analyses identified sensory perception and nucleoside diphosphate phosphorylation as the top affected terms for THs and E2, respectively. Using classic and additional RNA-seq-derived TH-response gene transcripts, we queried TH-disrupting activity in municipal wastewater effluent from two different treatment systems: anaerobic membrane bioreactor (AnMBR) and membrane enhanced biological phosphorous removal (MEBPR). While we observed physical EDC removal in both systems, some TH disruption activity was retained in the effluents. This work lays an important foundation for linking TH-dependent gene expression with olfactory system function in amphibians.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 202, September 2018, Pages 46-56
Journal: Aquatic Toxicology - Volume 202, September 2018, Pages 46-56
نویسندگان
Kevin W. Jackman, Nik Veldhoen, Rachel C. Miliano, Bonnie J. Robert, Linda Li, Azadeh Khojasteh, Xiaoyu Zheng, Tristan S.M. Zaborniak, Graham van Aggelen, Mary Lesperance, Wayne J. Parker, Eric R. Hall, Gregory G. Pyle, Caren C. Helbing,