کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8892201 | 1628543 | 2017 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
جنگلداری
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![عکس صفحه اول مقاله: A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening](/preview/png/8892201.png)
چکیده انگلیسی
Plant homeobox domain (PHD)-type transcription factors (TFs) are involved in a variety of biological processes. However, its involvement in commercially important fruit ripening process remains largely unclear. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that MaPHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. MaPHD1 was found localized in the nucleus and exhibited trans-repression ability. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay (EMSA) and transient expression analysis demonstrated that MaPHD1 directly bound to the G-rich motifs in the promoter of MaXTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripening, at least in part, by the direct suppression of MaXTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Horticultural Plant Journal - Volume 3, Issue 5, September 2017, Pages 190-198
Journal: Horticultural Plant Journal - Volume 3, Issue 5, September 2017, Pages 190-198
نویسندگان
WEI Wei, FAN Zhongqi, CHEN Jianye, KUANG Jianfei, LU Wangjin, SHAN Wei,