کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8892260 | 1628550 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of Citrus yellow vein clearing virus by Quantitative Real-time RT-PCR
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
جنگلداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To develop a rapid and reliable detection method for Citrus yellow vein clearing virus (CYVCV), a quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) system based on SYBR Green I was established by using a pair of specific primers designed from its conserved coat protein gene. The sensitivity, specificity, and applicability of the system were evaluated accordingly. The results showed that amplicons were produced from CYVCV isolates, whereas no amplicons from non-CYVCV citrus virus samples, including Citrus tristeza virus (CTV) and Citrus tatter leaf virus (CTLV), were obtained. The sensitivity of the qRT-PCR was 100-fold higher than that of conventional RT-PCR. An excellent linear correlation (R2â=â0.999) was obtained from two standard curves of cRNA, and the amplification efficiency was 102%. The data from field citrus samples detection showed that the qRT-PCR system could be used in determining the concentration of CYVCV in different citrus species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Horticultural Plant Journal - Volume 2, Issue 4, July 2016, Pages 188-192
Journal: Horticultural Plant Journal - Volume 2, Issue 4, July 2016, Pages 188-192
نویسندگان
CHEN Hongming, ZHOU Yan, WANG Xuefeng, ZHOU Changyong, YANG Xiuyan, LI Zhongan,