کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8892889 | 1628765 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Callus induction and establishment of cell suspension cultures of the halophyte Armeria maritima (Mill.) Willd.
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The objective of this work was to establish a cell suspension culture from Armeria maritima (Mill.) Willd., an halophyte so far only valuable for horticultural purposes, as a pre-requisite step towards the production of bio-actives secondary metabolites in vitro. For the initiation of callogenesis, five types of explants were tested: leaves from mature plants; leaves, cotyledons and roots from axenic seedlings; seeds. Three criteria either from a quantitative (percentage of callus induction) or a qualitative basis (aspect and friability) were used to evaluate the effectiveness of callus formation. Seeds-derived calli grown in the dark on MS medium supplemented with 4.5â¯Î¼M 2.4D and 0.93â¯Î¼M KIN were successfully selected for the establishment of cell suspension cultures, characterized by a growth index of 3.4 after 14â¯days of culture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 233, 15 March 2018, Pages 407-411
Journal: Scientia Horticulturae - Volume 233, 15 March 2018, Pages 407-411
نویسندگان
L. Gourguillon, C. Rustenholz, A. Lobstein, L. Gondet,