Optimizing factors affecting development and propagation of Bletilla striata in a temporary immersion bioreactor system

Culturing factors regarding to B. striata plantlets growth and pseudobulb development through temporary immersion bioreactor system (TIBS) were studied. Immersion frequency, sucrose concentration and inoculant amount were analyzed for their influence on seedling development. An immersion frequency of 3 min per 2 h was found to substantially promote the development of pseudobulbs. Seedling growth and development were also affected by the immersion frequency. The best seedling development was observed among those cultures that were immersed at a rate of 3 min per 6 h. Similarly, sucrose concentration was identified to affect the development of seedlings and pseudobulb formation obviously, with the best development recorded at 40 g/L weight ratio. A prominent seedling development, in terms of stem diameter, plant height and leaf width, were observed when 300 explants was loaded in the TIBS tank. The development of pseudobulbs was also found to be obviously enhanced at this explants loadings. The current findings verified the optimal immersion frequency, sucrose concentration and explant loads for improved seedling growth and pseudobulb development strategy for B. striata.