کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8895483 | 1630326 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of Silver Nanoparticles on Soil Microbial Communities and Bacterial Nitrification in Suburban Vegetable Soils
ترجمه فارسی عنوان
اثر نانوذرات نقره بر جوامع میکروبی خاک و نیتروفاسیون باکتریال در حوضه های سبز حومه
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کلمات کلیدی
باکتری اکسید کننده آمونیاک، نکروز سلولی، سمیت مسمومیت، یون نقره، نرخ بقا،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش خاک شناسی
چکیده انگلیسی
Silver nanoparticles (AgNPs) are widely used antimicrobial compounds; however, they may pose a threat to non-targeted bacteria in the environment. In this study high-throughput sequencing was used to investigate the effects of different concentrations of AgNPs (10, 50, and 100 mg kgâ1) on soil microbial community structure during short-term (7 d) exposure. The amounts of Acidobacteria, Actinobacteria, Cyanobacteria, and Nitrospirae significantly decreased with increasing AgNP concentration; meanwhile, several other phyla (e.g., Proteobacteria and Planctomycetes) increased and dominated. Nitrosomonas europaea, a well-characterized ammonia-oxidizing bacterium, was used to study the sensitivity of bacteria to AgNPs and ionic silver (Ag+). Flow cytometry was used to monitor the toxicity of low (1 mg Lâ1), middle (10 mg Lâ1), and high concentrations (20 mg Lâ1) of AgNPs, as well as Ag+ (1 mg Lâ1) released into the medium from 20 mg Lâ1 concentration of AgNPs, towards N. europaea. After 12 h of exposure, the survival rate of N. europaea treated with 1 mg Lâ1 Ag+ was significantly lower than those treated with low (1 mg Lâ1) and middle concentrations (10 mg Lâ1) of AgNPs, but the survival rate in the treatment with high concentration (20 mg Lâ1) of AgNPs was much lower. Additionally, necrosis rates were higher in the treatment with 20 mg Lâ1 AgNPs. Electron microscopy showed that Ag+ caused serious damage to the cell wall of N. europaea, disintegrated the nucleoids, and condensed next to the cell membrane; however, dissolved Ag+ is only one of the antibacterial mechanisms of AgNPs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pedosphere - Volume 27, Issue 3, June 2017, Pages 482-490
Journal: Pedosphere - Volume 27, Issue 3, June 2017, Pages 482-490
نویسندگان
Juan WANG, Kunhui SHU, Li ZHANG, Youbin SI,