کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8949921 | 1645726 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Digital image analysis of pan-cytokeratin stained tumor slides for evaluation of tumor budding in pT1/pT2 colorectal cancer: Results of a feasibility study
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کلمات کلیدی
pan-cytokeratinLNMIQRhpfICCROIImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیDigital image analysis - تجزیه و تحلیل تصویر دیجیتالinterobserver agreement - توافق بینابزارEMT - تکنسین فوریتهای پزشکیTumor budding - جوانه زدن تومورinter quartile range - دامنه بین دامنهColorectal cancer - سرطان روده بزرگLymph node metastases - متاستاز گره لنفاویregion of interest - منطقه مورد نظرhigh power field - میدان قدرت بالاHematoxylin and Eosin - هماتوکسیلین و ائوزینCRC - کد افزونگی دورهای Epithelial-mesenchymal transition - گذار اپیتلیال-مزانشیمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Manual assessment of tumor budding using pan-cytokeratin stained tumor slides exhibited high correlations (Spearman Rank 0.84-0.89, pâ¯<â¯0.001),excellent agreement between observers (Intra-class correlation coefficient (ICC): 0.86 -0.87) and 2.20 higher odds for regional metastases with increasing budding counts (pâ¯=â¯0.017). Digital image analysis correlated well to manual assessment (Spearman Rank 0.71-0.88) and agreement between the two methods was good (ICC 0.62-0.82). However, only a trend towards increased odds for metastatic progression was found for the adjusted digital estimates (pâ¯=â¯0.076). Digital estimates were higher than manual estimates, demonstrated by a systematic median difference of 3-4.5 buds. Image analysis was highly reproducible between users of the algorithm (ICC 0.98). In conclusion, assessment of tumor budding using pan-cytokeratin stained tumor slides is a method with high correlation and agreement between observers. Digital image analysis quantifies budding tumor cells in high agreement with manual estimates, but approval of the digital slides by a pathologist is mandatory. The method qualifies for further investigation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pathology - Research and Practice - Volume 214, Issue 9, September 2018, Pages 1273-1281
Journal: Pathology - Research and Practice - Volume 214, Issue 9, September 2018, Pages 1273-1281
نویسندگان
Rikke Karlin Jepsen, Louise Laurberg Klarskov, Michael Friis Lippert, Guy Wayne Novotny, Tine Plato Hansen, Ib Jarle Christensen, Estrid Høgdall, Lene Buhl Riis,