کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8950950 | 1645799 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of ghrelin O-acyltransferase inhibitor on gastric H+-K+-ATPase activity and GOAT/ghrelin system in gastric mucosal cells in vitro
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
Ghrelin is implicated in the regulation of gastric functional development. The octanoylation of ghrelin is critical for its physiological functions which dependent upon ghrelin O-acyltransferase (GOAT) catalyzation. To investigate the effect of GOAT on gastric acid secretion and expression of ghrelin in vitro. Primary cultures of gastric mucosal cells were challenged with 1.5â¯Ãâ¯10â5, 1.5â¯Ãâ¯10â4 and 1.5â¯Ãâ¯10â3â¯mol/mL GO-CoA-Tat (The GOAT inhibitor), respectively, for 24â¯h in order to further clarify the effect of GOAT on H+-K+-ATPase activity. In vitro, GO-CoA-Tat significantly increased ghrelin and GOAT mRNA expression at 1.5â¯Ãâ¯10â5, 1.5â¯Ãâ¯10â4 and 1.5â¯Ãâ¯10â3â¯mol/mL, and augmented cell total ghrelin secretion at 1.5â¯Ãâ¯10â3â¯mol/mL. But cell acylated ghrelin secretion was reduced at 1.5â¯Ãâ¯10â3â¯mol/mL GO-CoA-Tat (Pâ¯<â¯0.05). And cell acylated ghrelin synthesis was reduced at 1.5â¯Ãâ¯10â4 and 1.5â¯Ãâ¯10â3â¯mol/mL GO-CoA-Tat (Pâ¯<â¯0.05). In accordance with acylated ghrelin level, H+-K+-ATPase activity were decreased with 1.5â¯Ãâ¯10â4 and 1.5â¯Ãâ¯10â3â¯mol/mL GO-CoA-Tat (Pâ¯<â¯0.05). These results indicated that GOAT inhibitor decreases the acylated ghrelin level and H+-K+-ATPase activity in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 267, 1 October 2018, Pages 167-171
Journal: General and Comparative Endocrinology - Volume 267, 1 October 2018, Pages 167-171
نویسندگان
Gai Mei Du, Bi Ping Luo, Zhi Hua Hu, Jie Ge Wu, Wen Mei Yan, Zheng Qiang Han, Yu Hong Zhang, Mao Jun Liu,