کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8960662 | 1646423 | 2018 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular homeostatic tension and force transmission measured in human engineered tendon
ترجمه فارسی عنوان
تنش هیستوستات سلولی و انتقال نیروی اندازه گیری شده در تاندون مهندسی انسان
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کلمات کلیدی
تعامل سلول-ماتریس، فیبروبلاست، مانیتور نیروی مکانیک، مهندسی بافت،
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
چکیده انگلیسی
Tendons transmit contractile muscular force to bone to produce movement, and it is believed cells can generate endogenous forces on the extracellular matrix to maintain tissue homeostasis. However, little is known about the direct mechanical measurement of cell-matrix interaction in cell-generated human tendon constructs. In this study we examined if cell-generated force could be detected and quantified in engineered human tendon constructs, and if glycosaminoglycans (GAGs) contribute to tendon force transmission. Following de-tensioning of the tendon constructs it was possible to quantify an endogenous re-tensioning. Further, it was demonstrated that the endogenous re-tensioning response was markedly blunted after interference with the cytoskeleton (inhibiting non-muscle myosin-dependent cell contraction by blebbistatin), which confirmed that re-tensioning was cell generated. When the constructs were elongated and held at a constant length a stress relaxation response was quantified, and removing 27% of the GAG content of tendon did not alter the relaxation behavior, which indicates that GAGs do not play a meaningful role in force transmission within this system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 78, 10 September 2018, Pages 161-165
Journal: Journal of Biomechanics - Volume 78, 10 September 2018, Pages 161-165
نویسندگان
Antonis Giannopoulos, Rene B. Svensson, Katja M. Heinemeier, Peter Schjerling, Karl E. Kadler, David F. Holmes, Michael Kjaer, S. Peter Magnusson,