کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8967263 1101405 2005 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Acquisition of luteolytic capacity involves differential regulation by prostaglandin F2α of genes involved in progesterone biosynthesis in the porcine corpus luteum
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Acquisition of luteolytic capacity involves differential regulation by prostaglandin F2α of genes involved in progesterone biosynthesis in the porcine corpus luteum
چکیده انگلیسی
Luteolytic capacity is defined as the ability of corpora lutea (CL) to undergo luteolysis after prostaglandin (PG) F2α treatment. The mechanisms causing acquisition of luteolytic capacity are not yet identified but CL without luteolytic capacity have PGF2α receptors and respond to PGF2α with some changes in gene expression. Inhibition of progesterone biosynthesis is a key feature of luteolysis and therefore we postulated that genes involved in progesterone biosynthesis would be regulated by PGF2α differently in CL with or without luteolytic capacity. Gilts on day 9 after estrus (lack luteolytic capacity) or day 17 of pseudopregnancy (with luteolytic capacity) were treated with saline or a PGF2α analog (cloprostenol) and CL were collected 0.5 (Experiment I) or 10 h (Experiment II) later. In Experiment III, large luteal cells from CL on day 9 or 17 were cultured for 1, 12 and 24 h with or without PGF2α. PGF2α decreased LDL receptor mRNA (27%), steroidogenic acute regulatory protein (StAR) mRNA (41%), StAR protein (75%), LH receptor mRNA (55%), and LH receptor protein (45%) at 10 h after treatment in day 17 but not day 9 CL. PGF2α increased DAX-1 mRNA at 0.5 h (43%) and 10 h (46%) after PGF2α in day 17 but not day 9 CL but decreased 3βHSD mRNA (∼20% at 10 h) in both days 9 and 17 CL. In vitro, PGF2α decreased StAR mRNA at 12 h only in day 17 luteal cells; however, continuous treatment with PGF2α for 24 h decreased StAR mRNA in both days 9 and 17 luteal cells. Thus, luteolytic capacity involves a critical change in responsiveness of DAX-1, StAR, and LH receptor to PGF2α that results in inhibition of luteal progesterone biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Domestic Animal Endocrinology - Volume 28, Issue 2, February 2005, Pages 172-189
نویسندگان
, ,