کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987796 | 1555137 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue
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کلمات کلیدی
mRNART-PCREotaxinEBPMCPcDNA - cDNAComplementary DNA - DNA تکمیلیReverse transcriptase PCR - PCR معکوس رونویسیmessenger RNA - RNA messengerBALF - بافتRespiratory tract - دستگاه تنفسیReal-time RT-PCR - زمان واقعی RT-PCRCanine - سگCytokine - سیتوکینBronchoalveolar lavage fluid - مایع لارو برونکلوفلورmonocyte chemotactic protein - پروتئین chemotactic monocytethreshold cycle - چرخه آستانهChemokine - کموکاین یا کموکین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined in the respiratory mucosa of normal dogs or dogs with naturally acquired respiratory inflammation. In the present study, real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays were employed to quantify messenger RNA (mRNA) encoding the chemokines monocyte chemotactic protein (MCP)-2, eotaxin-2 and eotaxin-3, and the cytokines interleukin-4 (IL-4), IL-5, IL-6, IL-10, IL-12p40, IL-18, interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β) in normal nasal, bronchial and pulmonary tissues from puppies (n = 4) and from adult dogs (n = 7). There was no significant difference in the expression of any transcript between puppies and adult dogs at any of the anatomical sites examined. The expression of mRNA encoding eotaxin-2 and eotaxin-3 increased significantly with progression from the nasal mucosa to pulmonary parenchyma but expression of MCP-2 mRNA did not show this trend. At all levels of the respiratory mucosa, the most abundant transcripts were those encoding IL-18 and TGF-β. Transcripts encoding IL-6, IL-10, IL-12 and TNF-α were approximately ten-fold less abundant, and IL-4, IL-5 and IFN-γ were the least abundant templates. There was significantly different amount of mRNA encoding IL-5, IL-18 and TNF-α between particular anatomical levels of the respiratory mucosa while the mRNA expression of the other cytokines was similar at all anatomical sites. The results of the present study will enable comparisons to be made with results obtained from similar samples obtained from dogs with nasal, bronchial or pulmonary diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 104, Issues 3â4, 8 April 2005, Pages 195-204
Journal: Veterinary Immunology and Immunopathology - Volume 104, Issues 3â4, 8 April 2005, Pages 195-204
نویسندگان
D. Peeters, I.R. Peters, F. Farnir, C. Clercx, M.J. Day,